f Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter
- Authors: Ervin Fodor, Baik L. Seong†, George G. Brownlee
- J. Gen. Virol., July 1993 74: 1327-1333, doi: 10.1099/0022-1317-74-7-1327
- Subject: Animal
- Published Online:
A previous study of the 12 nucleotide-long influenza A virion RNA promoter has shown that three nucleotides, residues 9 to 11, were crucial for transcription in vitro, although other nucleotides play a significant but less important role. A model for polymerase-promoter recognition was proposed, according to which there were two sites: a binding site at residues 9 to 11 and a regulatory site at or near the site of initiation at residue 1. By studying the effect of point mutations in the promoter on the binding efficiency of the polymerase using a photochemical cross-linking assay, we now show that residues 9 to 12 are crucial for binding. In addition residues 4 to 8, though not as important, are involved in binding, possibly by stabilizing the polymerase-promoter complex. Both PB1 and PB2 apparently play an important role during virion RNA promoter recognition and binding.
Present address: Hanhyo Institute of Technology, Seoul, Korea.
© Society for General Microbiology 1993 | Published by the Society for General Microbiology
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