f Neutralizing Mechanisms of Two Human Monoclonal Antibodies Against Human Cytomegalovirus Glycoprotein 130/55
- Authors: Yoshifumi Ohizumi, Hiroshi Suzuki, Yoh-Ichi Matsumoto, Yasuhiko Masuho, Yoshio Numazaki
- J. Gen. Virol., October 1992 73: 2705-2707, doi: 10.1099/0022-1317-73-10-2705
- Subject: Animal
- Published Online:
The neutralization of human cytomegalovirus (HCMV) after adsorption to the cell surface at 4 °C was studied using two neutralizing monoclonal antibodies (C-23 and C-41) recognizing glycoprotein 130/55. HCMV adsorbed to cells was neutralized by C-23 (complement-independent), but not by C-41 (complement-dependent). Furthermore, the virus remained sensitive to C-23 for 120 min after shifting up from 4 °C to 37 °C, suggesting that C-23 might block an early stage of virus penetration into cells, and also that transition from virus attachment to virus penetration might be quite slow. The cell-to-cell infection of HCMV was also blocked only by C-23, and not by C-41. On the basis of the results presented here, we suggest that C-41 blocks the attachment of virus to the cell surface whereas C-23 prevents the penetration of virus into the cell.
© Society for General Microbiology 1992 | Published by the Society for General Microbiology
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