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Originally published as JGV in Press, 10.1099/vir.0.012724-0 on July 8, 2009 J Gen Virol 90 (2009), 2569-2574; DOI 10.1099/vir.0.012724-0

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Short Communication

Protective effect of the T112 PrP variant in sheep challenged with bovine spongiform encephalopathy

G. C. Saunders1, I. Lantier2, S. Cawthraw1, P. Berthon2, S. J. Moore3, M. E. Arnold4, O. Windl1, M. M. Simmons3, O. Andréoletti5, S. Bellworthy3 and F. Lantier2

1 Molecular Pathogenesis and Genetics Department, Veterinary Laboratories Agency (VLA Weybridge), New Haw, Addlestone, Surrey KT15 3NB, UK
2 INRA, UR1282, Infectiologie Animale et Santé Publique, F-37380 Nouzilly, France
3 Pathology Department, VLA Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK
4 CERA, VLA Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK
5 UMR INRA-ENVT, Interactions Hôtes–Agents Pathogènes, Ecole Vétérinaire de Toulouse, F-310761 Toulouse, France

Correspondence
S. Cawthraw
s.cawthraw{at}vla.defra.gsi.gov.uk

Sheep with an ARQ/ARQ PRNP genotype at codon positions 136/154/171 are highly susceptible to experimental infection with bovine spongiform encephalopathy (BSE). However, a number of sheep challenged orally or intracerebrally with BSE were clinically asymptomatic and found to survive or were diagnosed as BSE-negative when culled. Sequencing of the full PRNP gene open reading frame of BSE-susceptible and -resistant sheep indicated that, in the majority of Suffolk sheep, resistance was associated with an M112T PRNP variant (TARQ allele). A high proportion (47 of 49; 96 %) of BSE-challenged wild-type (MARQ/MARQ) Suffolk sheep were BSE-infected, whereas none of the 20 sheep with at least one TARQ allele succumbed to BSE. Thirteen TARQ-carrying sheep challenged with BSE are still alive and some have survival periods equivalent to, or greater than, reported incubation periods of BSE in ARR/ARR and VRQ/VRQ sheep.

A supplementary table detailing the ovine PRNP ORF PCR amplification conditions used is available with the online version of this paper.






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