J Gen Virol Try IJSEM Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 89 (2008), 1371-1379; DOI 10.1099/vir.0.83595-0

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Pignolet, B.
Right arrow Articles by Bertagnoli, S.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pignolet, B.
Right arrow Articles by Bertagnoli, S.
Agricola
Right arrow Articles by Pignolet, B.
Right arrow Articles by Bertagnoli, S.

Safety and immunogenicity of myxoma virus as a new viral vector for small ruminants

Béatrice Pignolet1,2, Séverine Boullier1,2, Jacqueline Gelfi1,2, Marjorie Bozzetti1,2, Pierre Russo3, Eliane Foulon1,2, Gilles Meyer1,2, Maxence Delverdier1,2, Gilles Foucras1,2 and Stéphane Bertagnoli1,2

1 INRA, UMR 1225, F-31076 Toulouse, France
2 Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France
3 AFSSA LERPRA les Templiers, 105 route des Chappes, F-06902 Sophia Antipolis, France

Correspondence
Stéphane Bertagnoli
s.bertagnoli{at}envt.fr

Myxoma virus (MYXV), a leporide-specific poxvirus, represents an attractive candidate for the generation of safe and non-replicative vaccine vectors for other species. With the aim of developing new recombinant vaccines for ruminants, we evaluated the safety and the immunogenicity of recombinant MYXV in sheep. In vitro studies indicated that ovine primary fibroblasts were not permissive for MYXV and that infection of ovine peripheral blood mononuclear cells occurred at a low rate. Although non-specific activation significantly improved the susceptibility of lymphocytes, MYXV infection remained abortive. Histological and immunohistochemical examination at the inoculation sites revealed the development of an inflammatory process and allowed the detection of sparse infected cells in the dermis. In addition, inoculated sheep developed an antibody response directed against MYXV and the product of the transgene. Overall, these results provide the first line of evidence on the potential of MYXV as a viral vector for ruminants.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2008 by the Society for General Microbiology.