HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lowings, P. Articles by Paton, D. Search for Related Content PubMed PubMed Citation Articles by Lowings, P. Articles by Paton, D. Agricola Articles by Lowings, P. Articles by Paton, D.

Classical swine fever virus diversity and evolution

Department of Virology, Central Veterinary Laboratory (Weybridge), Addlestone, Surrey KT15 3NB, UK

By analysing the nucleotide sequence data generated from both the E2 (gp55) and the NS5B genes of classical swine fever virus (CSFV), in addition to previously published data from the 5'NCR, we were able to divide 115 CSFV isolates into two major groups, five subgroups and two disparate isolates. Further discrimination was possible by analysis of sequence data from the E2 region. The three sequencing based methods were compared to monoclonal antibody (MAb) typing and to limited restriction enzyme (RE) mapping. Although both MAb and RE methods confirmed the previous classification the resolution was inferior. We estimated an approximate evolution rate for CSFV from an analysis of the virus variation observed in a single geographical area over a 6 year period. Applying this proposed rate to each of our deduced CSFV subgroups enabled us to calculate the approximate dates of divergence for each subgroup.

^* Author for correspondence. Fax +44 1932 357239. e-mail plowings.cvl.wood@gtnet.gov.uk

Received 6 October 1995; accepted 23 January 1996.

This article has been cited by other articles:

				C.-H. Pan, M.-H. Jong, Y.-L. Huang, T.-S. Huang, P.-H. Chao, and S.-S. Lai Rapid detection and differentiation of wild-type and three attenuated lapinized vaccine strains of Classical swine fever virus by reverse transcription polymerase chain reaction J Vet Diagn Invest, July 1, 2008; 20(4): 448 - 456. [Abstract] [Full Text] [PDF]

				B. Kim, J-Y. Song, D-S. Tark, S-I. Lim, E-J. Choi, J. Kim, C-K. Park, B-Y. Lee, S-H. Wee, Y-C. Bae, et al. Feed contaminated with classical swine fever vaccine virus (LOM strain) can induce antibodies to the virus in pigs Vet Rec., January 5, 2008; 162(1): 12 - 17. [Abstract] [Full Text] [PDF]

				G. Risatti, L. Holinka, Z. Lu, G. Kutish, J. D. Callahan, W. M. Nelson, E. Brea Tio, and M. V. Borca Diagnostic Evaluation of a Real-Time Reverse Transcriptase PCR Assay for Detection of Classical Swine Fever Virus J. Clin. Microbiol., January 1, 2005; 43(1): 468 - 471. [Abstract] [Full Text] [PDF]

				G. R. Risatti, J. D. Callahan, W. M. Nelson, and M. V. Borca Rapid Detection of Classical Swine Fever Virus by a Portable Real-Time Reverse Transcriptase PCR Assay J. Clin. Microbiol., January 1, 2003; 41(1): 500 - 505. [Abstract] [Full Text] [PDF]